Changes between Version 51 and Version 52 of SOPs/qc_shortReads


Ignore:
Timestamp:
11/19/19 14:40:42 (5 years ago)
Author:
twhitfie
Comment:

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  • SOPs/qc_shortReads

    v51 v52  
    114114}}}
    115115
     116In order to '''download a list of SRA files''' from NCBI, it is convenient to use prefetch.  Given a set of SRA files listed in a single column in the text file "sraFileList.txt" (e.g. SRR7623010, SRR7623011, etc.), the following command will download the entire set:
     117
     118{{{
     119prefetch --option-file sraFileList.txt
     120}}}
     121
     122This is current as of prefetch v. 2.9.3 (2.9.3-1).  Note that the default location for downloaded files is in your home directory under ~/ncbi/ncbi_public/sra.  With this default, one can quickly run out of space.  One solution to address this problem is to edit your ~/.ncbi/user-settings.mkfg file to include the following line:
     123{{{
     124/repository/user/main/public/root = "/destination/for/big/storage/here"
     125}}}
    116126
    117127== FastQC ==